Absolute Versus Relative Quantification
Absolute quantification converts Cq to copy number or concentration using a standard curve of known material (or bypasses curves entirely with digital PCR partitioning), while relative quantification reports a target's expression as a ratio to a reference gene and a calibrator sample. Relative methods cancel many sample-preparation errors but can never state how many molecules were present.
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Absolute quantification converts Cq to copy number or concentration using a standard curve of known material (or bypasses curves entirely with digital PCR partitioning), while relative quantification reports a target's expression as a ratio to a reference gene and a calibrator sample. Relative methods cancel many sample-preparation errors but can never state how many molecules were present.
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