Amplicon Length and Placement
Amplicon length is chosen for the application: roughly 70-200 bp for qPCR efficiency and degraded templates, longer for cloning or sequencing full features. Placement must also avoid repeats, SNPs under the primer sites and homopolymer runs.
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Amplicon length is chosen for the application: roughly 70-200 bp for qPCR efficiency and degraded templates, longer for cloning or sequencing full features. Placement must also avoid repeats, SNPs under the primer sites and homopolymer runs.
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