Amplification Efficiency from Standard Curve Slope
Efficiency is computed as E = 10^(-1/slope), with E = 2 (100% efficiency) corresponding to a slope of -3.32, meaning a 3.32-cycle shift per tenfold dilution. Acceptable assays typically fall between 90% and 110% efficiency; slopes steeper than -3.6 signal poor amplification and shallower than -3.1 usually signal inhibition or a non-linear dilution series rather than superhuman chemistry.
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Efficiency is computed as E = 10^(-1/slope), with E = 2 (100% efficiency) corresponding to a slope of -3.32, meaning a 3.32-cycle shift per tenfold dilution. Acceptable assays typically fall between 90% and 110% efficiency; slopes steeper than -3.6 signal poor amplification and shallower than -3.1 usually signal inhibition or a non-linear dilution series rather than superhuman chemistry.
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