Analyzing an MPRA: Barcode Counts Allelic Skew and Activity Calls

Apply-level computation; the difference between an active element and an allelic effect.

Questions this Concept answers

Why must barcode counts be normalized before comparing an element's activity across alleles?

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The mpra User’s Guide

This guide explains how massively parallel reporter assay data are turned into activity numbers. It shows that each tested regulatory element is represented by several barcodes, that DNA and RNA coun…