Annealing Temperature Optimization
Annealing is typically run a few degrees below the lower primer Tm, trading yield against specificity, and tuned empirically with a gradient or touchdown program. Too low invites mispriming; too high loses product entirely.
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Annealing is typically run a few degrees below the lower primer Tm, trading yield against specificity, and tuned empirically with a gradient or touchdown program. Too low invites mispriming; too high loses product entirely.
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