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Base Editing

A catalytically impaired Cas9 nickase fused to a deaminase chemically converts one base to another within a small editing window -- C to T for cytosine base editors, A to G for adenine base editors -- with no double-strand break and no donor template.

Questions this Concept answers

  • Why does nicking the strand opposite the edited base help make the chemical edit permanent, rather than the deaminase's action alone?