BCA Protein Assay
Peptide bonds reduce Cu2+ to Cu+ in alkaline conditions, and bicinchoninic acid chelates Cu+ to give a purple complex read at 562 nm against a BSA standard curve. It tolerates detergents such as SDS and Triton, unlike Bradford, but is inhibited by reducing agents like DTT and by chelators such as EDTA.
This Concept is waiting for its first lesson!
Peptide bonds reduce Cu2+ to Cu+ in alkaline conditions, and bicinchoninic acid chelates Cu+ to give a purple complex read at 562 nm against a BSA standard curve. It tolerates detergents such as SDS and Triton, unlike Bradford, but is inhibited by reducing agents like DTT and by chelators such as EDTA.
Are you a teacher? Sign in to start contributing.
Sign In