Beer-Lambert Law
Absorbance is proportional to molar absorptivity, path length and concentration, so a measured absorbance at a known wavelength gives concentration. Fmoc resin loading is quantified exactly this way from the dibenzofulvene-piperidine adduct at 301 nm.
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Absorbance is proportional to molar absorptivity, path length and concentration, so a measured absorbance at a known wavelength gives concentration. Fmoc resin loading is quantified exactly this way from the dibenzofulvene-piperidine adduct at 301 nm.
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