Cell Lysis Buffer Selection
Lysis buffer strength is chosen against the target's subcellular location: non-ionic detergents such as NP-40 keep complexes intact for cytoplasmic proteins, while RIPA's ionic SDS and deoxycholate are needed to release nuclear and membrane-embedded targets at the cost of disrupting protein-protein interactions.
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Lysis buffer strength is chosen against the target's subcellular location: non-ionic detergents such as NP-40 keep complexes intact for cytoplasmic proteins, while RIPA's ionic SDS and deoxycholate are needed to release nuclear and membrane-embedded targets at the cost of disrupting protein-protein interactions.
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