Cell Versus Debris Gate
The first gate on an FSC-A versus SSC-A plot, drawn to exclude the low-FSC cloud of membrane fragments, RBC ghosts, and free nuclei that would otherwise dilute every downstream frequency. Debris is excluded by position, not by a threshold that would also discard small live lymphocytes.
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The first gate on an FSC-A versus SSC-A plot, drawn to exclude the low-FSC cloud of membrane fragments, RBC ghosts, and free nuclei that would otherwise dilute every downstream frequency. Debris is excluded by position, not by a threshold that would also discard small live lymphocytes.
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