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CRISPR Off-Target Effects

Cas9 tolerates mismatches between guide and DNA, especially in the PAM-distal region, so it cuts unintended loci that resemble the target. Off-target editing is measured empirically by genome-wide assays rather than predicted from sequence alone.

D

Demerzel Video

CRISPR Off-Target Effects

Cas9 tolerates mismatches between guide and DNA, especially in the PAM-distal region, so it cuts unintended loci that resemble the target. Off-target editing is measured empirically by genome-wide as…