Degenerate Primer Design
A mixed-base primer synthesized with IUPAC ambiguity positions covers all codon variants of a conserved motif in unsequenced or divergent templates. Every added degenerate position multiplies the pool and dilutes each species, so degeneracy is kept low and away from the 3' end.
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A mixed-base primer synthesized with IUPAC ambiguity positions covers all codon variants of a conserved motif in unsequenced or divergent templates. Every added degenerate position multiplies the pool and dilutes each species, so degeneracy is kept low and away from the 3' end.
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