Deparaffinization and Rehydration
Xylene or a xylene substitute dissolves the paraffin, then descending alcohols to water restore an aqueous environment so water-based reagents can reach the tissue. Residual wax from incomplete clearing produces patchy, focally absent staining.
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Xylene or a xylene substitute dissolves the paraffin, then descending alcohols to water restore an aqueous environment so water-based reagents can reach the tissue. Residual wax from incomplete clearing produces patchy, focally absent staining.
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