Designing a Pooled CRISPR Screen: Library Coverage and Controls

Idea sketch 3 entry point; the design decisions that determine whether a screen can find anything.

Questions this Concept answers

Why do pooled screens include non-targeting and safe-harbor control guides in the library?

jeremy Text

Genome-wide CRISPR-Cas9 knockout screens

A pooled CRISPR knockout screen transduces one vessel of cells in bulk with a lentiviral library of single guide RNAs covering every gene, then selects for a phenotype and sequences the surviving gui…