Sanger sequencing operates on the principle of chain-termination synthesis to determine nucleotide sequences by generating a population of DNA fragments with specific lengths corresponding to each base position. The method utilizes differential electrophoretic mobility in an ordered matrix to separate these terminators, where fluorescent labeling distinguishes cytosine (green), adenine (blue), guanine (yellow), and thymine (red) for automated detection. This biochemical mechanism serves as a foundational high-throughput technique within molecular biology for resolving primary nucleotide structures of genetic material.
Sanger sequencing operates on the principle of chain-termination synthesis to determine nucleotide sequences by generating a population of DNA fragments with specific lengths corresponding to each ba…