EMSA and Allele-Specific ChIP for Differential Transcription Factor Binding

Enrichment; direct tests of the motif-disruption mechanism.

Questions this Concept answers

Why is allele-specific ChIP used to test whether a variant disrupts transcription factor binding in vivo?

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Interpreting EMSA Gel Shifts for DNA-Protein Binding in Molecular Biology

The electrophoretic mobility shift assay (EMSA) is a molecular biology technique for detecting DNA-protein interactions by exploiting the principle that a DNA-protein complex migrates more slowly thr…