Enzyme Assay Design
A valid assay fixes pH with an adequate buffer and fixes temperature, keeps enzyme far below substrate, spans [S] roughly from 0.2 to 5 times Km, and verifies that signal is linear in both time and enzyme concentration before any constant is reported.
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A valid assay fixes pH with an adequate buffer and fixes temperature, keeps enzyme far below substrate, spans [S] roughly from 0.2 to 5 times Km, and verifies that signal is linear in both time and enzyme concentration before any constant is reported.
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