Enzyme-Conjugated Secondary Antibody
An anti-species secondary carrying multiple HRP or alkaline phosphatase molecules binds the primary's constant region, amplifying signal several-fold per bound primary. The secondary must match the primary's host species and, for multiplexing, be cross-adsorbed against the other species present to prevent species cross-reactivity.
This Concept is waiting for its first lesson!
An anti-species secondary carrying multiple HRP or alkaline phosphatase molecules binds the primary's constant region, amplifying signal several-fold per bound primary. The secondary must match the primary's host species and, for multiplexing, be cross-adsorbed against the other species present to prevent species cross-reactivity.
Are you a teacher? Sign in to start contributing.
Sign In