Epitope Masking by Cross-Linking
Methylene bridges lock protein conformation and physically occlude or distort antibody binding sites, and calcium-protein complexes formed during fixation add further steric blockade. The epitope is usually intact but inaccessible, which is why retrieval recovers rather than recreates signal.
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Methylene bridges lock protein conformation and physically occlude or distort antibody binding sites, and calcium-protein complexes formed during fixation add further steric blockade. The epitope is usually intact but inaccessible, which is why retrieval recovers rather than recreates signal.
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