Exon-Junction-Spanning Primers
Placing a primer across a spliced exon-exon junction, or the two primers in different exons flanking a large intron, makes genomic DNA either unamplifiable or distinguishable by product size. This is the design-level control against gDNA contamination in RT-qPCR.
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Placing a primer across a spliced exon-exon junction, or the two primers in different exons flanking a large intron, makes genomic DNA either unamplifiable or distinguishable by product size. This is the design-level control against gDNA contamination in RT-qPCR.
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