Exponential Amplification Assumption in qPCR
All Cq-based quantification assumes product accumulates as N0 * E^n during the phase where the threshold is crossed, which makes Cq linear in log(N0). Inhibitors, primer-dimer competition, and secondary structure break this assumption by making E vary between samples, which is why efficiency must be measured rather than assumed.
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All Cq-based quantification assumes product accumulates as N0 * E^n during the phase where the threshold is crossed, which makes Cq linear in log(N0). Inhibitors, primer-dimer competition, and secondary structure break this assumption by making E vary between samples, which is why efficiency must be measured rather than assumed.
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