Fluorescence Bleed-Through
Signal from one fluorophore appearing in the detection channel intended for another, caused by overlapping emission tails or by one laser line cross-exciting the wrong dye. It is diagnosed with single-label control samples and reduced by sequential line-by-line scanning and narrower detection bands.
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Signal from one fluorophore appearing in the detection channel intended for another, caused by overlapping emission tails or by one laser line cross-exciting the wrong dye. It is diagnosed with single-label control samples and reduced by sequential line-by-line scanning and narrower detection bands.
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