Fluorescence Minus One Control
A sample stained with every antibody in the panel except the one being measured, revealing exactly how much spillover-driven spread reaches that empty channel. The FMO is the control that legitimately sets a positivity gate; unstained and isotype controls do not, because they omit the panel-wide spread that shifts the true negative boundary.
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A sample stained with every antibody in the panel except the one being measured, revealing exactly how much spillover-driven spread reaches that empty channel. The FMO is the control that legitimately sets a positivity gate; unstained and isotype controls do not, because they omit the panel-wide spread that shifts the true negative boundary.
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