Fluorescence Panel Design
Assigning fluorophores to markers so that the brightest dyes carry the dimmest or most-continuous antigens, and so that highly co-expressed markers are not placed on dye pairs with large spillover spreading. Good assignment is decided from a spreading matrix and antigen density, not from whichever conjugates are in the freezer.
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Assigning fluorophores to markers so that the brightest dyes carry the dimmest or most-continuous antigens, and so that highly co-expressed markers are not placed on dye pairs with large spillover spreading. Good assignment is decided from a spreading matrix and antigen density, not from whichever conjugates are in the freezer.
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