Fluorescence Spillover
Fluorophore emission spectra are broad and overlapping, so a dye assigned to one detector also deposits photons in others; the fraction of a dye's signal landing in a secondary detector is fixed by optics and is a property of the dye-detector pair, not of the sample.
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Fluorophore emission spectra are broad and overlapping, so a dye assigned to one detector also deposits photons in others; the fraction of a dye's signal landing in a secondary detector is fixed by optics and is a property of the dye-detector pair, not of the sample.
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