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Hematoxylin Counterstaining

A light hematoxylin counterstain renders nuclei blue so the chromogenic signal can be localized to a cell compartment and the section can be assessed morphologically. Over-counterstaining masks weak DAB, and in fluorescence DAPI or Hoechst plays the equivalent role.

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A light hematoxylin counterstain renders nuclei blue so the chromogenic signal can be localized to a cell compartment and the section can be assessed morphologically. Over-counterstaining masks weak DAB, and in fluorescence DAPI or Hoechst plays the equivalent role.

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