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Hi-C Chromatin Conformation Capture

How crosslinking and proximity ligation turn three-dimensional contact in the nucleus into read pairs that join loci megabases apart in the linear sequence.

Questions this Concept answers

Why does Hi-C require a restriction digestion or similar fragmentation step before ligation?

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Hi-C Chromosome Conformation Capture in Chromatin Biology

Hi-C is a chromosome conformation capture technique in molecular biology that maps the three-dimensional spatial organization of chromatin genome-wide by cross-linking, fragmenting, and re-ligating c…