Lekkala Vinod Reddy PhD
41 minutes
High-Fidelity Cas9 Variants
Variants such as SpCas9-HF1, eSpCas9, and HiFi Cas9 remove non-specific protein-DNA contacts so the enzyme is trapped in an inactive conformation when the guide is mismatched. Delivering Cas9 as a short-lived ribonucleoprotein further limits exposure time.