High-Fidelity Cas9 Variants
Variants such as SpCas9-HF1, eSpCas9, and HiFi Cas9 remove non-specific protein-DNA contacts so the enzyme is trapped in an inactive conformation when the guide is mismatched. Delivering Cas9 as a short-lived ribonucleoprotein further limits exposure time.
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Variants such as SpCas9-HF1, eSpCas9, and HiFi Cas9 remove non-specific protein-DNA contacts so the enzyme is trapped in an inactive conformation when the guide is mismatched. Delivering Cas9 as a short-lived ribonucleoprotein further limits exposure time.
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