Immunohistochemistry Troubleshooting Logic
Absent signal is triaged through control behavior: positive control also negative points at retrieval, detection reagent or protocol failure, while a working positive control points at pre-analytics or true negativity in the test tissue. High background is separated into diffuse (insufficient block, over-concentrated primary), edge and drying artifact, and structured background (endogenous enzyme, biotin, Fc binding).
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Absent signal is triaged through control behavior: positive control also negative points at retrieval, detection reagent or protocol failure, while a working positive control points at pre-analytics or true negativity in the test tissue. High background is separated into diffuse (insufficient block, over-concentrated primary), edge and drying artifact, and structured background (endogenous enzyme, biotin, Fc binding).
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