In-Silico PCR Specificity Checking
Both primers are searched against the target genome or transcriptome to enumerate every pair of opposing sites within amplifiable distance, weighting 3'-end matches most heavily. A design is accepted only when exactly one such predicted amplicon exists.
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Both primers are searched against the target genome or transcriptome to enumerate every pair of opposing sites within amplifiable distance, weighting 3'-end matches most heavily. A design is accepted only when exactly one such predicted amplicon exists.
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