Lentiviral Delivery and Multiplicity of Infection in Pooled Screens

Apply-level: compute the fraction of cells with exactly one guide from an MOI.

Questions this Concept answers

Why do pooled CRISPR screens use a low multiplicity of infection, typically around 0.3?

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Genome-wide CRISPR-Cas9 knockout screens

Genome-wide CRISPR-Cas9 knockout screens introduce a library of single guide RNAs into a cell population, almost always using third-generation lentiviral vectors that stably integrate into the host g…