Live-Cell Confocal Imaging
Imaging under maintained temperature, humidity, and CO2 while spending the smallest photon budget that answers the question, typically by opening the pinhole slightly, using sensitive detectors, and accepting a noisier image over a dead cell. Sampling interval is set by the biological process rate, and healthy-control morphology should be checked against the imaged field.
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Imaging under maintained temperature, humidity, and CO2 while spending the smallest photon budget that answers the question, typically by opening the pinhole slightly, using sensitive detectors, and accepting a noisier image over a dead cell. Sampling interval is set by the biological process rate, and healthy-control morphology should be checked against the imaged field.
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