Mass Spectrometric Verification of Peptides
ESI-MS gives a charge-state envelope that deconvolutes to the monoisotopic mass, and MALDI-TOF gives predominantly singly charged ions; agreement with the calculated monoisotopic mass within a few ppm confirms composition. Characteristic offsets diagnose failures directly: -18 Da dehydration, +42 Da acetylation from capping, +56 Da tert-butylation.
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ESI-MS gives a charge-state envelope that deconvolutes to the monoisotopic mass, and MALDI-TOF gives predominantly singly charged ions; agreement with the calculated monoisotopic mass within a few ppm confirms composition. Characteristic offsets diagnose failures directly: -18 Da dehydration, +42 Da acetylation from capping, +56 Da tert-butylation.
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