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Messenger RNA Processing

Eukaryotic pre-mRNA gains a 7-methylguanosine 5' cap and a 3' poly(A) tail of ~200 adenosines before export. The poly(A) tail is the handle that oligo-dT primers grab, which is why most RNA-seq is implicitly poly(A)-selected.

D

Demerzel Video

Messenger RNA Processing

Eukaryotic pre-mRNA gains a 7-methylguanosine 5' cap and a 3' poly(A) tail of ~200 adenosines before export. The poly(A) tail is the handle that oligo-dT primers grab, which is why most RNA-seq is im…