Microtome Sectioning of Paraffin Blocks
A microtome cuts 3-5 um ribbons that are floated on a warm water bath and picked up onto charged or silane-coated slides so sections survive retrieval. Section thickness directly changes apparent stain intensity, so it must be held constant across a comparison set.
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A microtome cuts 3-5 um ribbons that are floated on a warm water bath and picked up onto charged or silane-coated slides so sections survive retrieval. Section thickness directly changes apparent stain intensity, so it must be held constant across a comparison set.
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