Nearest-Neighbor Melting Temperature Calculation
Tm is computed from summed dH and dS of each adjacent dinucleotide plus initiation terms, then corrected for oligo and salt concentration via Tm = dH / (dS + R ln(Ct/4)). It is the accurate method and the one primer software reports.
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Tm is computed from summed dH and dS of each adjacent dinucleotide plus initiation terms, then corrected for oligo and salt concentration via Tm = dH / (dS + R ln(Ct/4)). It is the accurate method and the one primer software reports.
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