No-Reverse-Transcriptase Control
The no-RT control runs the full reaction on RNA with reverse transcriptase omitted, so any amplification must come from contaminating genomic DNA rather than cDNA. Interpreting it requires a Cq gap of several cycles from the +RT reaction; the durable fixes are DNase treatment of the RNA and intron-spanning primer design.
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The no-RT control runs the full reaction on RNA with reverse transcriptase omitted, so any amplification must come from contaminating genomic DNA rather than cDNA. Interpreting it requires a Cq gap of several cycles from the +RT reaction; the durable fixes are DNase treatment of the RNA and intron-spanning primer design.
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