No-Template Control and Primer-Dimer Detection
The no-template control replaces template with water and should never cross threshold; amplification in it means contamination or, more often, primer-dimer formed by primers annealing to each other. Primer-dimers melt at a lower temperature than the amplicon and consume reagents, so with dye chemistry they inflate apparent signal and compress the assay's low-copy sensitivity.
This Concept is waiting for its first lesson!
The no-template control replaces template with water and should never cross threshold; amplification in it means contamination or, more often, primer-dimer formed by primers annealing to each other. Primer-dimers melt at a lower temperature than the amplicon and consume reagents, so with dye chemistry they inflate apparent signal and compress the assay's low-copy sensitivity.
Are you a teacher? Sign in to start contributing.
Sign In