Paraffin Tissue Processing and Embedding
Fixed tissue is dehydrated through graded alcohols, cleared in xylene, infiltrated with molten paraffin and oriented in a block, because water and wax are immiscible. Excessive heat or prolonged processing during infiltration causes additional protein denaturation and further epitope loss.
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Fixed tissue is dehydrated through graded alcohols, cleared in xylene, infiltrated with molten paraffin and oriented in a block, because water and wax are immiscible. Excessive heat or prolonged processing during infiltration causes additional protein denaturation and further epitope loss.
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