PCR Amplification Efficiency
Efficiency is derived from the slope of Cq versus log template across a serial dilution as E = 10^(-1/slope) - 1, with 90-110% and R-squared above 0.99 the acceptance window. Comparative quantification is invalid when assays being compared differ in efficiency.
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Efficiency is derived from the slope of Cq versus log template across a serial dilution as E = 10^(-1/slope) - 1, with 90-110% and R-squared above 0.99 the acceptance window. Comparative quantification is invalid when assays being compared differ in efficiency.
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