PCR Primer Function
A short synthetic oligonucleotide anneals to one strand and supplies the free 3'-OH the polymerase extends from, with forward and reverse primers pointing toward each other. The pair defines both the boundaries and the strand orientation of the product.
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A short synthetic oligonucleotide anneals to one strand and supplies the free 3'-OH the polymerase extends from, with forward and reverse primers pointing toward each other. The pair defines both the boundaries and the strand orientation of the product.
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