Peptide Coupling Cycle
Each residue is added by a fixed loop: Nalpha-deprotection, wash, coupling of the next activated Fmoc-amino acid, wash, and an optional capping step with acetic anhydride to terminate deletion sequences. Because yield compounds over the chain, a 99% per-cycle efficiency still leaves only about 74% of a 30-mer intact.
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Each residue is added by a fixed loop: Nalpha-deprotection, wash, coupling of the next activated Fmoc-amino acid, wash, and an optional capping step with acetic anhydride to terminate deletion sequences. Because yield compounds over the chain, a 99% per-cycle efficiency still leaves only about 74% of a 30-mer intact.
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