Perturb-seq: Single-Cell Transcriptome Readout of Pooled Perturbations

Enrichment; the screen readout that also identifies target genes and pathways.

Questions this Concept answers

Why can Perturb-seq identify both a hit gene and its downstream pathway in a single experiment?

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Perturb-seq Analysis in Single-Cell Functional Genomics

Perturb-seq (broadly, single-cell pooled CRISPR screening) combines pooled genetic perturbation screens with high-content single-cell RNA sequencing so that each individual cell's transcriptome is jo…