Pooled Screen Readouts: Growth FACS Sorting on a Reporter and Sequencing Guides

The design of the selection step; determines what a hit means.

Questions this Concept answers

Why does the choice between a growth-based screen and a FACS-reporter screen change what counts as a 'hit'?

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Using Illumina Sequencing to Identify Hits in Genome-Wide CRISPR Knockout Screens

CRISPR-Cas9 is an RNA-guided nuclease system in which a short guide RNA directs the Cas9 protein to a complementary genomic target site (adjacent to a required protospacer-adjacent motif) to introduc…