Primer 3' End Specificity
Mismatches within the last few 3' bases block extension, while 5' mismatches are largely tolerated, so specificity is concentrated at the 3' terminus. Any off-target site with a matching 3' end is a real mispriming risk regardless of overall identity.
This Concept is waiting for its first lesson!
Mismatches within the last few 3' bases block extension, while 5' mismatches are largely tolerated, so specificity is concentrated at the 3' terminus. Any off-target site with a matching 3' end is a real mispriming risk regardless of overall identity.
Are you a teacher? Sign in to start contributing.
Sign In