Primer-Dimer Formation
Self-dimers between identical primers and hetero-dimers between the two primers create extendable duplexes that amplify as short products and consume reagents. 3'-end complementarity is the dangerous case because it is directly extendable.
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Self-dimers between identical primers and hetero-dimers between the two primers create extendable duplexes that amplify as short products and consume reagents. 3'-end complementarity is the dangerous case because it is directly extendable.
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