Primer GC Content
A GC fraction of about 40-60% keeps Tm in a workable range and avoids AT-rich primers that anneal weakly or GC-rich primers that form stable off-target and structured duplexes. GC content sets the coarse stability; sequence order sets the fine detail.
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A GC fraction of about 40-60% keeps Tm in a workable range and avoids AT-rich primers that anneal weakly or GC-rich primers that form stable off-target and structured duplexes. GC content sets the coarse stability; sequence order sets the fine detail.
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