Primer Hairpin Structure
A primer with an internal self-complementary segment folds into a stem-loop that sequesters it from the template. Hairpins with a dG near or below about -3 kcal/mol at the annealing temperature, especially involving the 3' end, should be redesigned out.
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A primer with an internal self-complementary segment folds into a stem-loop that sequesters it from the template. Hairpins with a dG near or below about -3 kcal/mol at the annealing temperature, especially involving the 3' end, should be redesigned out.
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