Primer Length Selection
Primers of roughly 18-25 nucleotides are long enough that their sequence is statistically unique in a mammalian genome yet short enough to anneal fast and synthesize cleanly. Longer primers gain little specificity while losing annealing kinetics and synthesis yield.
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Primers of roughly 18-25 nucleotides are long enough that their sequence is statistically unique in a mammalian genome yet short enough to anneal fast and synthesize cleanly. Longer primers gain little specificity while losing annealing kinetics and synthesis yield.
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