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Primer Pair Experimental Validation

A new pair is accepted only after a single band of expected size on a gel, a single melt-curve peak, no-template and no-RT controls clean, and an efficiency within the acceptance window. Computational design predicts behavior; only these controls confirm it.

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A new pair is accepted only after a single band of expected size on a gel, a single melt-curve peak, no-template and no-RT controls clean, and an efficiency within the acceptance window. Computational design predicts behavior; only these controls confirm it.

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